神经肽P物质与烫伤创面愈合关系的实验研究

Experimental study on the relationship between neuropeptide substance P and wound healing in scalded rats

目的 探讨神经肽P物质(SP)与烫伤创面愈合之间的关系。 方法 (1)制作大鼠不同深度烫伤模型,分别于伤后1、3、7、14d致死,用放射免疫法测定创面SP含量。(2)将大鼠肉芽组织成纤维细胞(GTF)用不同培养液培养,分为空白对照组、SP组及SP+SP受体拮抗剂(Spantide)组。体外检测SP及Spantide对GTF增殖活性[以吸光度(A)值表示]及凋亡率的影响。 结果 (1)伤后1d,浅Ⅱ、深Ⅱ、Ⅲ度烫伤创面SP含量分别为(145±78)、(94±48)、(53±27)ng/g,深Ⅱ度创面与其余两者比较,差异有统计学意义(P<0 01).浅Ⅱ度创面伤后3、7dSP含量显著增高;深Ⅱ度创面伤后7、14dSP含量显著增高;Ⅲ度创面伤后SP含量无显著变化。(2)SP增强GTF增殖活性(空白对照组A为0. 21±0. 05,SP组A为0. 36±0 07,P<0. 01)并抑制其凋亡,Spantide可抑制SP对GTF的作用。 结论 SP可促进GTF增殖,创面SP含量与创面损伤程度及愈合能力关系密切。

Objective To explore the relationship between neuropeptide substance P (SP) and wound healing in scalded rats. Methods (1) Scalded rats with different degrees of scald injury were employed as the experimental model and were sacrificed at 24 post scald hour (PSH), and on 3, 7 and 14 post scald days (PSD). The SP content in the wound was detected with radioimmunoassay method. (2) The murine granulation tissue fibroblasts(GTF) were cultured with different culture media, and divided into control, SP and Spantide(SP receptor antagonism) groups. The effects of SP and Spantide on the cellular activity and apoptotic rate of murine GTF were assessed in vitro. Results There was significant difference of the SP content among the superficial (145±78) ng/g, partial (94±48 ng/g) and full thickness (53±27 ng/g) scald wounds at 24 PSH ( P <0.01), while the SP content in partial thickness burn wound on 3 and 7 PSD obviously increased; and that in deep partial thickness burn wound obviously increased on 7 and 14 PSD. But the SP content remained unchanged in full thickness scald wound. (2) SP could promote the activity of GTF and inhibit its apoptosis (The GTF activity in control,SP groups were 0.21±0.05,0.36±0.07,respectively, P <0.01). Spantide could inhibit the interaction between SP and GTF. Conclusion SP can promote GTF proliferation, and the SP content in wound is closely associated with the depth of the injury and wound healing capacity.[