卫星RNA SatC382对辅助病毒的影响

The effects of a satellite RNA of Cucumber mosaic virus on its helper virus

通过体外转录将黄瓜花叶病毒(CMV)卫星RNASatC382与不带卫星的黄瓜花叶病毒CNa株系进行假重组,获取带卫星的CMV重组株(CNa -SatC382)。经dsRNA提取及RT PCR检测,证实CNa和SatC382在假重组株中能稳定共存。测定CNa和CNa -SatC382的14种寄主生物学反应,并统计两者接种昆诺藜、假酸浆、心叶烟、西葫芦7、14、21、28d的病情指数,结果显示:带卫星和不带卫星的CMV在各供试寄主上表现的症状差别不明显,病情指数也无显著差异。由此推测卫星RNASatC382没有改变辅助病毒CMV -CNa株系对寄主症状的影响。

Pseudorecombination of a Cucumber mosaic virus CNa strain (CNa) and a satellite RNA, CNa-SatC382,was obtained by in vitro transcription. The results showed that CNa and CNa-SatC382 can coexist steadily in Nicotiana glutinosa by dsRNA extraction, RT-PCR and sequencing. CNa-SatC382 was inoculated on 14 different hosts and the disease index of Chenopodium quinoa, Nicandra physanodes, N. glutinosa and Cucurbita pepo in 7,14,21,28 days post-inoculation was analyzed. The results showed that CNa either with or without satellite imposed no obvious influence on disease symptom of all tested host plants or disease index. In short, SatC382 had no influence on host symptom caused by helper virus CMV-CNa.