塞替派诱发人支气管上皮恶性转化成瘤细胞的生物学特性

Biological characteristics of tumorigenic human bronchial epithelial cells induced by thiotepa

目的 为进一步阐明化学致癌剂的作用机制提供实验依据。方法 利用原代细胞培养,细胞免疫化学分析获得并鉴定恶性转化成瘤细胞,以BEAS 2B细胞和BEAS STE细胞为参照,研究恶性转化成瘤细胞的增殖动力学和锚着独立生长能力等表型特征。结果和结论 经原代细胞培养获得可传代的具人类上皮来源的恶性转化成瘤细胞。

AIM To provide the experimental evidences for explaining the mechamism of chemical tumorigenesis. METHODS Three tumorigenic cell lines derived from tumor tissues were established. Using the BEAS-2B and BEAS-STE cells as control, the dynamics of proliferation and anchorage independence growth of the BEAS-TT cells were studied. RESULTS and CONCLUSION The proliferation rate of the three BEAS-TT cells is slower than BEAS-2B and BEAS-STE cells. The anchorage independence growth capacity of BEAS-TT cells is stronger than BEAS-STE.