利用PC-3M-1E8细胞亚系建立人前列腺癌淋巴道转移模型

Establishment of a lymph node metastatic model with human prostate cancer cell subclone PC-3M-1E8

目的 建立裸鼠人前列腺癌淋巴道转移模型以便用于转移机制和控制方法的研究。方法 分别采用原位接种及皮下接种两种方法将高转移性前列腺癌细胞亚系PC 3M 1E8接种于裸小鼠体内,4 0d后检测其成瘤情况及局部淋巴结自发性转移情况。结果 原位接种法的裸小鼠成瘤率及淋巴结转移率均达1 2 / 1 2 ,皮下接种法的裸小鼠成瘤率也达1 2 /1 2 ,但未检测到任何淋巴结转移。结论 对该细胞株采用原位接种法建立裸鼠人前列腺癌淋巴结转移模型获得成功。

AIM To establish a lymph node metastatic model for studying the metastatic mechanism and experimental therapy of prostate cancer. METHODS After human prostate cancer cell subclone PC-3M-1E8 was inoculated under orthotopic or ectopic environments in nude mice, we employed gross and histological examinations to determine tumorigenicity and the production of lymph node metastasis. RESULTS PC-3M-1E8 cells were equally tumorigenic with 12/12 frequency in the prostate and subcutis. They exhibited highly metastatic potentiality with 12/12 lymph node metastasis after orthotopic implantation in nude mice for 40 d, whereas showed no lymph node metastasis after ectopic implantation in nude mice. CONCLUSION The results demonstrated that establishment of a lymph node metastatic model using orthotopic implantation in nude mice is successful, and it may become a valuable model for the study of metastatic mechanism and experimental therapy of prostate cancer.