高效液相色谱测定大鼠心肌组织腺苷酸含量

High performance liquid chromatogram (HPLC) determination of adenosine phosphates in rat myocardium

目的:优化高效液相色谱(HPLC)法检测大鼠心肌组织腺苷酸的含量.方法:ODS HYPERSIL C18分析柱,检测波长254 nm,流动相为50 mmol/L磷酸钾缓冲液(pH 6.5).结果:心肌三磷酸腺苷(ATP)、二磷酸腺苷(ADP)和一磷酸腺苷(AMP)平均回收率分别为99%～107%,96%～104%和95%～119%;日内和日间相对标准偏差(RSD)分别在1.5%和5.1%以下.结论:优化高效液相色谱法快速准确、简便易行.

Objective: To establish method for the quantitative determination of adenosine phosphates in rat myocardium by optimized high performance liquid chromatogram (HPLC). Methods: ODS HYPERSIL C 18 column and a mobile phase of 50 mmol/L tribasic potassium phosphate buffer solution (pH 6.5), with UV detector at 254 nm were used. Results: The average recovery rates of myocardial adenosine triphosphate (ATP), adenosine diphosphate(ADP)and adenosine monophosphate (AMP) were 99%-107%, 96%-104% and 95%-119%, respectively;relative standard deviations(RSDs)of within-day and between-days were less than 1.5% and 5.1%, respectively. Conclusion: The method is simple, rapid and accurate, and can be used to analyse the adenosine phosphates in myocardium.