用共聚焦显微镜研究plk1在秋水仙胺和长春新碱抗肿瘤效应中的作用

Role of plk1 in the anti-cancer effect of colcemid and vincri stine against K562 cells

目的:研究plk1在秋水仙胺和长春新碱抗肿瘤效应中的作用。方法:采用Westernblotting和共聚焦显微镜检测秋水仙胺、长春新碱及plk1反义寡核苷酸(Asodn)处理对K5 6 2细胞plk1及γ微管蛋白表达的影响。结果:Westernblotting检测结果提示秋水仙胺和长春新碱不影响plk1和γ微管蛋白的表达量,但共聚焦显微镜观察发现秋水仙胺和长春新碱影响plk1在分裂期聚集和中心体形成。plk1Asodn处理不仅可明显降低plk1蛋白表达,而且影响γ微管蛋白聚合形成中心体。结论:秋水仙胺和长春新碱抗肿瘤效应可能是通过plk1介导的抑制γ微管蛋白聚合形成中心体来实现的,plk1具有作为肿瘤治疗靶点的可能性。

AIM: To study the role of plk1 in the ant i-cancer effect of colcemid and vincristine against K562 cells. METHODS: K562 cells were treated with colcemid and vincristine a nd antisense oligonucleotide of plk1, then expression of plk1 and γ-tubulin wer e investigated by Western blotting and confocal microscopy. RESULTS: Treatment of K562 cells with colcemid and vincristine i nfluenced the condensation of plk1 and assembly of γ-tubulin, though without ch ange of protein quantity. Treatment with antisense oligonucleotide of plk1 not o nly reduced the expression of plk1 without influence on protein quantity of γ-t ubulin detected by Western blotting, but also disturbed the formation of centros ome observed by confocal microscopy. CONCLUSION: The function of colcemid and vincristine destructing the spindle might be realized through the mechanism of restraining condensation of plk1 and assembly of γ-tubulin, which might be dependent on plk1. plk1 may be a potential target in anti-cancer therapy.