衰老对小鼠精子成熟和受精能力的影响

Effects of aging on sperm maturation and fertility in mice

目的:探讨衰老对附睾精子成熟、体外受精和胚胎发育的影响。方法:取老龄小鼠(18月龄,n =15 )和青年小鼠(6月龄,n =15 )附睾头精子和附睾尾精子,分别检测精子密度、存活率、活动率、正常形态率和胞浆小滴率,并通过体外受精比较附睾尾精子受精率和各阶段胚胎发育率。结果:老龄小鼠附睾头精子和附睾尾精子活动率、精子密度显著低于青年组(P <0 . 0 1) ,胞浆小滴率和畸形精子率显著高于青年组(P <0 .0 5 ) ,附睾尾精子的受精率下降(P <0 .0 1) ,胚胎各阶段发育率降低(P <0 . 0 1)。结论:衰老影响小鼠精子功能及附睾精子成熟过程。小鼠可作为雄性生殖衰老研究的动物模型。

AIM: To investigate the effects of aging on sperm maturation and fertility in mice. METHODS: Sperm of caput epididymides and cauda epididymides were obtained from Kunming mice aged 6 months (n=15, as control) and 18 months ( n=15). Sperm parameters including sperm density, viability, motility and nor mal morphological rate were recorded. Sperm of cauda epididymides was observed b y transmission electron microscope. The fertility potential and embryo developme ntal competence were performed by in vitro fertilization and embryo culture. RESULTS: Sperm motility, density and normal morphological rate i n aged mice were lower than those in control (P<0.05). The fertilization ra te and embryo developmental rate of aged group were significantly lower than tho se in control (P<0.01). CONCLUSION: Aging influences spermatogenesis in testes and sperm maturation in epididymides. Mouse would be served as a good model for male repr oductive aging research.