电针促进帕金森病模型小鼠黑质致密部突触可塑性的神经细胞黏附机制

Neuron adhesion mechanism in substantia nigra compacta of Parkinson's disease mice by electroacupuncture therapy correlated to dopaminergic neural plasticity

目的探讨神经细胞黏附分子在电针促进帕金森病小鼠黑质致密部(SNC)突触可塑性中的作用。方法以1甲基4苯基1,2,3,6四氢吡啶(MPTP)诱导的帕金森病模型小鼠作为研究对象,电针“合谷”、“太冲”穴,每日1次,7次为1疗程,共3个疗程后,分别运用免疫组化和原位杂交检测神经细胞黏附分子及其mRNA表达。结果免疫组化阳性细胞计数分别为模型组(54.00±10.39),电针组(92.67±2.52)(P<0.05);原位杂交阳性细胞计数分别为模型组(138.33±5.86),电针组(230.33±17.16)(P<0.05)。结论电针可促进帕金森病模型小鼠SNC神经细胞黏附分子及其mRNA的表达,从而促进帕金森病模型小鼠突触可塑性的发挥。

Objective To explore the effect of neural cell adhesion molecule(NCAM)on dopaminergic plasticity of substantia nigra compacta (SNC) in Parkinson's disease (PD) mice enhanced by electroacupuncture (EA). Methods PD model mice induced by 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine were as subjects. Hegu and Taichong were punctured once daily, 7 times as one period. The expressions of NCAM and NCAM mRNA were determined by immunohistochemistry and in situ hybridization after three periods. Results The NCAM(92.67±2.52)and NCAM mRNA(230.33±17.16)positive cells in EA group were higher than those(54.00±10.39, 138.33±5.86)in model group (P<0.05, P<0.01).Conclusions EA could promote the expression of NCAM and NCAM mRNA in PD mice SNC and enhance degenerative dopaminergic neuron plasticity.