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大鼠局灶脑缺血预处理对caspase-3表达影响的研究 被引量：3

The influence of focal brain ischemic preconditioning in rat on expression of caspase-3
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摘要 目的观察对比caspase3在大鼠局灶脑缺血预处理和脑缺血再灌注中的表达。方法采用线栓法局灶脑缺血模型,ABC免疫组化方法检测caspase3表达。结果caspase3免疫反应阳性细胞数缺血预处理组(IPC+MCAO)明显低于单纯局灶缺血组(SS+MCAO),阳性细胞呈棕褐色,清晰可辨,IPC+MCAO组caspase3蛋白阳性细胞在缺血6h表达开始增多,24h达高峰,72h下降;SS+MCAO组caspase3蛋白阳性细胞表达时相一致,但增高明显。结论缺血预处理可能通过降低caspase3表达,对缺血引起的神经损伤起到保护作用。 Objective To investigate and compare the differences in caspase-3 expressions after focal cerebral ischemic preconditioning (IPC) and cerebral ischemic reperfusion in rats. Methods Caspase-3 expressions in Longa' rat model for focal cerebral ischemia were determined by ABC immunohistochemistry technique. Results The positive caspase-3 immunoreactivity in IPC+MCAO group were fewer than that in SS+MCAO group, presented chocolate brown and identified clearly; that in IPC+MCAO group increased at 6 h after ischemia, reached peak at 24 h, reduced at 72 h; that of SS+MCAO group was same with significant increase. Conclusions Focal ischemic preconditioning might be play a protective role in neurological impairment induced by ischemia through decreasing caspase-3 expressions.
作者 康治臣 徐忠信 邬英全 王晶余 钱佳利
机构地区 吉林大学中日联谊医院神经内科 长春中医学院生理教研室
出处 《中国老年学杂志》 CAS CSCD 北大核心 2005年第4期414-415,共2页 Chinese Journal of Gerontology
关键词 CASPASE-3 缺血 预处理 再灌注 Caspase-3 Ischemia Preconditioning Reperfusion
分类号 R743 [医药卫生—神经病学与精神病学]
  • 相关文献

参考文献8

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同被引文献18

  • 1余晓慧.电针对局灶性脑缺血大鼠Caspase-3蛋白表达的影响[J].辽宁中医学院学报,2005,7(2):167-167. 被引量：9
  • 2牛敬忠,王贤军,夏青,王涛,夏作理.局灶性脑梗死大鼠半胱氨酸天冬氨酸蛋白酶3和9的表达:空间分布特征及时间演变规律[J].中国临床康复,2005,9(21):26-27. 被引量：1
  • 3滕军放,云琳,冯建玉,姜晓蕊,卢宏.局灶性脑缺血再灌注大鼠大脑皮质中Caspase-3蛋白的表达[J].郑州大学学报（医学版）,2005,40(5):838-839. 被引量：4
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引证文献3

二级引证文献21

中国老年学杂志
2005年 第4期

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