海马内注射叠氮钠对大鼠行为学及类P3的影响

Effects of injecting sodium azide into rat's hippocampus on its behavior and P3-like potential

目的观察在大鼠海马内微量注射叠氮钠(NaN3)对其行为学及类事件相关电位(类P3)的影响。方法SD大鼠随机分为叠氮钠海马损毁组及生理盐水对照组。前者分别在双侧海马CAI区微量注射质量分数5%NaN31μl,每天1次,连续3d。最后1次注射后第3d开始进行Morris水迷宫行为测定,之后检测类P3;最后进行脑组织形态学检查。结果与对照组比较,叠氮钠海马损毁组大鼠海马神经元内线粒体数量明显减少,并伴随线粒体固缩、出现空泡等现象;而水迷宫测试平均逃避潜伏期和认知电位类P3潜伏期显著延长(P<0.05);水迷宫测试错误次数则明显增多(P<0.05)。结论海马内分次微量注射NaN3可建立起一种相对快速、简便的能量代谢障碍性大鼠模型,且行为学及类P3的变化表明其有痴呆表现。

Objective To observe the effects of injecting sodium azide into rat's hippocampus on its behavior and P3-like potential. Methods 1 μl 5% of sodium azide was injected into bilateral CA1 of rat's hippocampus, 3 times daily for 3 days and morris water maze test was carried out at the 3rd day after the final injection to determine P3-like potential. Finally morphologic test was made. Results Compared with that of controls, the mitochondrion number of hippocampus neuron in model rats reduced obviously, and accompanied with pyknosis or vacuole; the model rats' average escape latency and P3-like latency prolonged and error numbers increased statistically (P<0.05). Conclusions The method of injecting sodium azide into the rat's hippocampus could simply and rapidly establish rat's model of energy metabolism dysfunction with the changes of behavior and P3-like potential indicating dementia to some extent.