熊果苷拮抗H_2O_2 损伤的研究(英文)

The study of arbutin against the damage caused by H_2O_2 in ECV-304 cells

目的 通过体外细胞学实验观察熊果苷拮抗H2 O2 的作用。方法 体外培养人脐静脉内皮细胞ECV 30 4 ,将细胞分为对照组、损伤组和熊果苷组,熊果苷组提前12h加入熊果苷,5 0 0 μmol/LH2 O2 诱导细胞氧化应激状态。光镜实验比较各组细胞形态学的变化;MTT实验比较各组细胞增殖活力的改变。结果 光镜下可见,对照组细胞结构清晰,呈单层铺路石状紧密排列;损伤组细胞变形、皱缩、排列紊乱、脱落明显,胞浆内出现空泡;熊果苷组细胞形态结构基本清晰,但细胞有轻度肿胀,其它接近于正常对照组;MTT结果显示,与损伤组相比,熊果苷能明显保护ECV- 30 4细胞增殖活力(P <0 .0 5 )。结论 本实验结果提示,熊果苷可以抵御H2 O2 所致ECV- 30 4细胞氧化应激损伤。

Objective To study the protection of arbutin against the damage caused by H_2O_2 in ECV-304 cells.Methods The cells were cultured in vitro and were divided into three groups:control group,damage group and arbutin group in which the cells were pretreated with 500μg/L arbutin, while the same amount of PBS was added to the other two groups. After 12h, the cells in damage group and arbutin group were treated with 500μmol/L H_2O_2. After 1h, light microscope and MTT were used to observe the change of the cells in three groups.Results Through light microscope, in control group, the cells grew in pebble-shaped arrangement. In damage group, the cells became out of shape and most of them peeled off. In arbutin group, the cells were in a better state which was similar to that of control group except the slight swelling of the cells; MTT assay showed significant difference between damage group and arbutin group (P<0.05). Conclusion Arbutin could protect ECV-304 cells from the damage caused by H_2O_2.