犬Elovl2基因片段的克隆和测序分析

Cloning And Sequence Analysis On Canine Elovl2 Gene Fragments

利用比较基因组的方法获得犬elongationofverylongchainfattyacids(FEN1/Elo2,SUR4/Elo3,yeast)-like2基因(Elovl2)序列,为后续研究奠定基础。根据人ELOVL2和小鼠的Elovl2基因序列设计PCR引物,对比格犬和本地犬的DNA进行PCR扩增,回收纯化扩增片段,克隆、测序。对本地犬和比格犬的三次克隆测序分别得到长为672bp、671bp和675bp的基因片段,三个基因片段有11个碱基差异。测序结果表明,犬的Elovl2基因与人的ELOVL2基因和小鼠的Elovl2基因无显著相似性。扩增得到的基因片段还需进一步分析。

To acquire the DNA sequence of canine Elovl2 gene using comparative genomics methods for subsequent research. Designing PCR primers according to human ELOVL2 and murrne Elovl2gene, the canine Elovl2 gene fragments were amplified with Beagle and domestic dogs' DNA by PCR method. Then these fragments were withdrawn, purified, cloned and sequenced. The results of three-time cloning and sequencing of canineElovl2 gene fragments were 672bp、671bp and 675bp, respectively. There were 11-base differences in these three fragments. The results of sequencing were not significantly similar with human ELOVL2 or house mouseElovl2 These canine fragments of amplification should be further analyzed.