火鸡传染性法氏囊病病毒YL株VP2基因的克隆和序列比较

Cloning and Sequence Comparison of the VP2 Gene of Infectious Bursal Disease Virus YL Strains Isolated from Turkey

参考GenBank发表的传染性法氏囊病毒(IBDV)基因组序列,设计并合成了一对特异扩增IB-DVVP2基因的引物。以广西玉林发病火鸡群分离的IBDV野毒YL株为材料,以其基因组为模板利用RT-PCR技术扩增出了1.5kb的cDNA产物,将VP2基因克隆于PUC119质粒上,得到重组PUC119质粒。并对VP2基因全序列测定、分析和聚类表明,YL株与欧洲超强毒株非常相似,而与经典强毒株、弱毒株和变异株相差较大。

According to the reported nucleotide sequence of IBDV in GenBank, a pair of primers that can amplify the cDNA of protective antigen VP2 gene was designed and synthesized. By RT-PCR, a single DNA fragment of about 1.5 kb was obtained from YL strain of IBDV isolated from turkey. Then the VP2 cDNA was cloned into PUC119 at Sma Ⅰ site. The nucleotide sequence of the expected VP2 gene was determined by Sanger's DNA sequencing method, and then the amino acid sequence was deduced. Both the nucleotide sequence and deduced amino acid sequence were compared with five published sequence of VP2 gene of IBDV strains. It shown that YL strain Is most closely related to the very virulent strain UK661 and OKYM, but different from other serotype Ⅰ strains.