韭菜栽培品种遗传多样性的ISSR和RAPD研究

Genetic Diversity of Cultivated Chinese Chive Germplasm by ISSR and RAPD Markers

利用RAPD(RandomAnaplifiedPolymorphicDNA)和ISSR(Inter-simpleSequenceRepeat)两种分子标记技术对20份韭菜栽培品种进行了遗传多样性研究。结果表明,筛选后选用的12个ISSR引物和15个RAPD引物分析分别产生了258和101条扩增产物带,其中多态性条带(即20个韭菜品种中一个或多个但不是全部具有的带)分别为132和40条,分别占总数的51.2%和39.6%。,也就是说12个ISSR引物和15个SSR引物对韭菜不同品种的扩增可分别产生51.2%和39.6%的多态性带。根据两种标记的结果,利用NTSYS软件计算Nei氏遗传距离,然后以Nei氏遗传距离矩阵按UPGMA方法进行聚类分析,发现供试材料之间具有较低的遗传多样性。其品种间遗传距离分别只有0.02-0.2和0.04-0.13,且大部分品种并没有按来源省份聚在一起。鉴于此,又对两种标记的多样性分析结果利用Mantel(1968)检验进行相关性检测,发现两种结果存在较低的相关性:r=0.47174,P<0.05。

Random amplified polymorphic DNA (RAPD) and Inter-simple sequence repeat (ISSR) molecular markers were used to analyze the genetic diversity of 20 cultivated Chinese chive's germplasm. The results are as fellows: Of the primers tested, 12 ISSR and 15 RAPD primers were selected for their reproducibility and high polymorphism. About 258 bands were obtained by 12 ISSR primers and 101 bands were obtained by 15 RAPD primers. Of which , the polymorphic bands are 132 and 40 ,the percent are 51.2 % and 39.6 % respectively. The data were analyzed by NYSTS program. Based on the data, the Nei's genetic distance is obtained by NTSYS program, then UPCMA cluster analysis is using by the Nei's genetic distance . The conclusion is that the tested materials have the inferior genetic diversity , and the genetic distance is 0.02-0.2 and 0.04-0.13 among the breeds respectively. Most of the materials aren't clustering by the provinces. Whereas , based on the correlation test gained by Mantel Test (1968) through analyzing the genetic diversity ,we can found that the two results have the lower correlation ;r=0.47174, P<0.05.