摘要
应用孕酮单克隆抗体（ＰＭｃＡｂ）和孕酮－辣根过氧化物酶（Ｐ－ＨＲＰ）通过微量板对猪血清孕酮进行酶免疫分析。结果表明：１：２．５×１０￣４浓度的ＰＭｃＡｂ和１：５×１０￣４浓度的Ｐ－ＨＲＰ之间具有较强的亲和力，母猪在发情配种前注射释放激素类似物（ＬＲＨ－Ａ＿３）能提高怀孕早期的孕酮含量，怀孕母猪在怀孕１５天时的孕酮含量是未孕猪的近２倍，分别为１７．２４ｎｇ／ｍＬ和９．５０８ｎｇ／ｍＬ。
An enzymeimmunoassay of swine progesterone was developed using progesterone monoclone antibody(PMcAb and progesterone-horseradish peroxidase (p-HRP)in the microtitreplates. There was a stronger affinity between (PMcAb ) and P-HRP when the concentrations were1:2.5× 10￣4 and 1 :5× 10￣4, respectively.An increasing progesterone concentration of serum in ear-ly pregnancy was found in the sows to whom luteinizing hormone analogue(LRH -A_3)had been ad-ministered before in semination in comparison with control animals,On the 15th day of pregnancy,progesterone concentration in pregnant sows was 17.24 ng/mL being 2 times that in non-pregnant sows(9. 58 ng/mL).
基金
浙江省科委资助
关键词
酶免疫分析
孕酮
释放激素
母猪
enzymeimmunoassay
progesterone
serum
LRH
sow
