双核钌(II)多吡啶配合物与酵母RNA的相互作用研究

Interaction of a Dinuclear Polypyridyl Ruthenium(II) Complex with Yeast-RNA

用紫外-可见吸收光谱和荧光光谱滴定、稳态荧光淬灭和反向盐滴定实验研究了双核钌(II)配合物[(bpy)2Ru(ebipcH2)Ru(bpy)2](ClO4)4 {bpy=2,2'-联吡啶; ebipcH2=N-乙基-4,7-二(咪唑-[4,5-f]-(1,10-邻菲啰啉)-2-基)咔唑}与酵母RNA 的相互作用. 结果表明该双核配合物以插入方式与酵母RNA 作用, 在生理盐浓度下(≈150 mmol/L NaCl)该配合物与RNA 的相互作用明显强于DNA.

The binding of a dinuclear Ru(II) complex of [(bpy)2Ru(ebipcH2)Ru(bpy)2](ClO4)4, where bpy= 2,2'-bipyridine and ebipcH2=N-ethyl-4,7-bis(imidazolo[4,5-f]-(1,10-phenanthrolin)-2-yl)carbazole, to yeast- RNA has been investigated by UV-visible and emission spectroscopy titrations, steady-state emission quenching and reverse salt titration experiments. The results suggest that the binding of this complex to the RNA be intercalative with a binding constant of 9.1×105 L/mol at 50 mmol/L NaCl, which is much stronger than to the DNA at physiological saline concentration (≈150 mmol/L NaCl).