可降解胶原蛋白-几丁聚糖三维骨架构建的实验研究

Experimental Research on Construction of 3-Dimen-sional Scaffolds with Absorbable Collagen-Chitosan

目的 研究以胶原蛋白几丁聚糖构建应用于血管组织工程学研究的可降解三维支架。方法 以不同配比Ⅰ型胶原蛋白和几丁聚糖为生物材料,体外预构具有三维结构的支架,观察各自的空间立体结构;通过动物皮下埋植降解和体外胰蛋白酶、溶菌酶和胶原酶溶液直接酶解,观察不同配比材料的降解特性及生物相容性。结果 构建的三维支架孔径均匀,生物相容性好。不同材料配比直接影响支架形态,几丁聚糖可以影响胶原酶对支架的降解,同时几丁聚糖掺入增加后也可减少体内降解。结论 构建的胶原蛋白几丁聚糖三维支架具有结构均匀、生物相容性好的特点,可以应用于血管组织工程研究。

Objective To investigate the feasibility for fabricating the 3-dimensional scaffolds with collagen-chitosan applied to tissue engineering of blood vessel. Methods Different proportion of collagen to chitosan suspensions were used as the biomaterials to construct 3-D scaffolds in vitro. And the 3-D structure in the scaffolds were observed. The scaffolds biodegradability and biocompatible property were observed by imbedding them subcutaneously or dipping in the solution of collagenase (Ⅰ), bacteriolysin, trypsinase solutions. Results The fabricated scaffolds had the relatively uniform structure, with perfect biocompatibility. The different proportions for collagen to chitosan could work on the construction character in scaffolds, the chitosan could influence collagenase degradation for scaffolds and prolong the degradation progress in vivo. Conclusion The 3-dimentional scaffolds made from collagen-chitosan, with relatively uniform and excellent biocompatibility, can be used in vascular tissue engineering research.