血浆不同水平胰岛素对大鼠动脉血压的影响

Different Plasma Insulin Levels on Systolic Blood Pressure of Rats

目的 探讨血浆不同水平胰岛素对大鼠动脉血压的影响。方法 将实验动物随机分为胰岛素泵组、四氧嘧啶组和正常对照组。采用胰岛素微量渗透泵皮下植入的方法建立外源性胰岛素诱导高胰岛素血症动物模型;给SD大鼠腹腔注射四氧嘧啶,建立低胰岛素血症和高血糖动物模型;分别于实验的0、2、5、8、11、15 d测定血浆胰岛素及血糖浓度,于实验的1、4、7、10、13、16 d测定动脉血压。结果 1)胰岛素微量渗透泵可诱导出明显的高胰岛素血症,与对照组相比差异有统计学意义(P<0.05)。2)实验第10天胰岛素泵组动脉血压增高,并持续至实验结束,与正常对照组和四氧嘧啶组相比差异有统计学意义(P<0.01),而正常对照组和四氧嘧啶组动脉血压波动不明显,四氧嘧啶组虽分别于实验第13、16 天观察到动脉血压稍有上升趋势,但与正常对照组相比差异无统计学意义(P>0.05)。结论 外源性胰岛素可诱导出大鼠动脉血压的上升,而在短期内未观察到低胰岛素血症和高血糖动物有明显的动脉血压变化。

Objective The purpose of study was to explore the effects of different plasma insulin levels on systolic blood pressure of rat model. Methods Experimental animals were divided into three groups randomly: insulin osmotic mini-pump group (induced-hyperinsulinemia); alloxan-induced diabetes group (induced-hypoinsulinemia & hyperglycemia) and normal control group (normal insulinemia). Hyperinsulinemia animal model was induced by injection of insulin subcutaneously through of a mini-pump, hypoinsulinemia and hyperglycemia animal models were induced by intraperitoneal injection of alloxan (40 mg/kg), and then the plasma insulin and glucose levels were measured on the experimental day of 0, 2, 5, 8, 11, 15, respectively, the systolic blood pressure was measured on the experimental day of 1, 4, 7, 10, 13, 16, respectively. Results The insulin osmotic mini-pump induced significant hyperinsulinemia compared with control group(P<0.05), and the significant systolic blood pressure uprising was observed on the experimental day of 10, untie the end of the experiment in the insulin osmotic mini-pump group.There was a significant difference between hyperisulinemia group and the others(P<0.01), otherwise, there was no obviously systolic blood pressure increasing in both control group and alloxan group, although the systolic blood pressure of alloxan group to be observed an increasing trend on the experimental day of 13 and 16, respectively, but there was no significant statistic meaning compared with control group(P>0.05). Conclusion Exogenous insulin can induce uprising of systolic blood pressure of rats; there is no significant variations of systolic blood pressure in model of hypoinsulinemia and hyperglycemia during the experimental period.