急性坏死性胰腺炎修复中胶原Ⅰ的表达和胰腺细胞的修复

Expression of Collagen TypeⅠand Repairment of Pancreatic Cell in Acute Necrotizing Pancreatitis

目的 探讨急性胰腺炎(AP)修复中胶原Ⅰ的表达和胰腺细胞的修复。方法 将NIHswiss小鼠分为2 组:盐水对照组和急性胰腺炎组。急性胰腺炎诱导采用蛙皮素腹腔注射,用常规病理评价胰腺的炎症程度;免疫组织化学方法检测胶原Ⅰ的表达;流式细胞仪检测细胞增生状态。结果 蛙皮素腹腔注射可诱导小鼠急性胰腺炎。急性胰腺炎组小鼠诱导后8 h胰腺组织损伤最严重,至第7天时组织损伤基本恢复正常。AP时胶原Ⅰ的表达增强。AP诱导后细胞增生状态分3个时期:早期的增生活跃期、中期的增生抑制期和后期的高增生状态。结论 AP是一种自限性疾病,机体可自行修复损伤。AP机体修复过程中,纤维化修复占重要地位,胶原Ⅰ表达升高;胰腺细胞增生在修复中也起重要作用。

Objective To investigate the expression of collagen type Ⅰ and the effect of pancreatic cell in repairment of acute pancreatitis(AP). Methods NIH swiss rats were divided into two groups: one was the control, the other was acute pancreatitis. Acute pancreatitis were induced by intraperitoneal injection of cerulein. Routine pathological examination was used to evaluate the degree to pancreac inflammation,the expression of collagen type Ⅰ was detected by method of immunohistochemistry, and the proliferation of cell was examined by flow cytometry analysis. Results Intraperitoneal of cerulein injection could induce AP, it was the most serious duration of injury of pancreas that 8 h after cerulein injection and recovered seven days later. Expression of collagen type Ⅰ was increased in AP, and three stages of cell proliferation after AP was induced: the early active stage and the middle inhibitive stage; the last high stage. Conclusion AP is a self-limited disease, which can be repaired by bodyself. Fibrosis of mesenchyma, increasing of expression of collagen type Ⅰ and proliferation of pancreatic cell play important role in the repairment of AP.