紫外分光光度法测定注射用前列腺素E_1的含量

Content Determination of Prostaglandins E_1 for Injection by UV

目的:采用碱异构化法对注射用前列腺素E1 进行含量测定。方法:紫外分光光度法,1.0 m ol·L- 1 氢氧化钾甲醇液为异构化反应溶媒,检测波长为2 78nm。结果:检测线性范围2～10 μg·ml- 1 ,r=0 .9999,平均回收率为99.8% ,RSD=0 .89%。结论:本法检测结果准确、可靠,可用于控制注射用前列腺素E1 的质量。

Objective:To establish the UV Spectrophotometry method of the determination of Prostaglandins E 1 for Injection by the method of isomer alkali.Methods:UV Spectrophotometry was used.Use 1.0mol·L -1 KOH methanol solution as isomeric menstruum.The detective wavelength was 278nm.Results:Linearity was obtained in the range of 2～10μg·ml -1 (r=0.9999).The average recovery was 99.8%,with RSD 0.89%(n=5).Conclusion:The method was accurate,simple,rapid and can be used to determine the Prostaglandins E 1 for Injection.