HPLC法测定复方止咳糖浆中盐酸异丙嗪和氢溴酸右美沙芬的含量

Determination of Chlorpromazine Hydrochloride and Dextromethorpham Hydrobromide in Compound Zhike Syrup by HPLC

目的:建立高效液相色谱法,测定复方止咳糖浆中盐酸异丙嗪和氢溴酸右美沙芬的含量。方法:采用C1 8柱,以0 .5 %三乙胺溶液(用磷酸调节p H值至3.5 ) -乙腈(75∶2 5 )为流动相,检测波长为2 78nm。结果:线性范围为盐酸异丙嗪 0 .1738-0 .86 90μg(r=0 .9999) ;氢溴酸右美沙芬0 .4 4 7- 2 .2 35μg(r=1.0 0 0 0 ) ,平均回收率为盐酸异丙嗪;10 0 .0 % (RSD% =0 .87% ,n= 9) ;氢溴酸右美沙芬99.8% (RSD=0 .3% ,n=9)。结论:本方法简便,快速,专属性强,可有效的控制复方止咳糖浆中盐酸异丙嗪和氢溴酸右美沙芬的含量。

Objective:An HPLC method was used for determination of chlorpromazine Hydrochloride and Dextromethorpham Hydrobromide in Compound Zhike Syrup.Methods: Using HPLC with Kromasil C 18 column,0.5%triethylamine(pH3.5 by phosphoric acid)-acetonitrile(75∶25)as the mobile phase.The wavelength used for detection was 278nm.Results:A better linearity and good correlation coefficient were obtained:Chlorpromazine Hydrochloride was 0.1738～0.8690μg(0.9999);dextromethorpham hydrobromide was 0.447～2.235μg(r=1.0000).The satisfactory recoveries were obtained:Chlorpromazine Hydrochloride was 100.0%(RSD%=0.87%);Dextromethorpham hydrobromide was 99.8%(RSD=0.3%).Conclusion:The method was convenient and accurate.It can be used as a method of quality control.