珊瑚羟基磷灰石结合血管内皮细胞生长因子修复兔骨缺损的实验研究

Use of coralline hydroxyapatite/vascular endothelial growth factor in the repair of segmental radius defects in rabbits

目的探讨珊瑚羟基磷灰石(CHA)结合血管内皮细胞生长因子(VEGF)修复骨缺损的效果,为其临床、科研应用提供实验依据。方法以CHA作为VEGF的可吸附性载体,制备成复合人工骨,将其植入兔尺骨中段10mm骨缺损处,以单纯CHA组,自体骨移植组和空白组作为对照,在术后2、4、8、12周,进行大体解剖、X线摄片、病理组织切片、生物力学测试等方法观察,研究各组骨愈合,血管化情况及力学强度。结果在2、4、8周病理组织切片及X线摄片显示骨缺损修复程度CHA/VEGF组明显优于自体移植骨组优于单纯CHA组,而空白组骨缺损处被纤维组织及肌组织等填充。生物力学测试显示术后12周CHA/VEGF组抗扭转强度明显优于自体骨移植组。结论CHA与VEGF结合有明显促进骨缺损修复的作用,强于自体骨移植及单纯CHA移植。二者协同,可用于骨缺损修复。

Objective To investigate the efficacy of combined use of vascular endothelial growth factor (VEGF)and coralline hydroxyapatite(CHA) for repairing segmental bony defects. Methods CHA/ VEGF composite was prepared and implanted in continuous defects in ulna of adult rabbits ,and CHA,autograft implantation or no graft were used as controls .The animals were sacrificed on 2,4,8 and 12 weeks after operation.The bone repair process was investigated using physical,roentgenographical,histological and biomechanical methods. Results The vascularization was better in CHA/ VEGF transplanted areas than in CHA,autograft implantation or control groups on 2,4 and 8 weeks after operation.Histological and roentgenographic examination showed that CHA/ VEGF composite used to the repairs was more rapid compared with CHA or autograft implantation,and the repair sites in the control group were filled with fibrous and muscular tissues.Biomechanic tests showed that on the CHA/ VEGF side the callus grew more quickly and vigorously,and the bone density was higher and the antibending force was stronger than autograft implantation after 12 weeks. Conclusion CHA/ VEGF can efficiently repair bony defects,and is the best material.