蓝光促进黑曲霉分生孢子发育和产糖化酶的研究

Effect of blue light on conidiation development and glucoamylase enhancement in Aspergillus niger

以黑暗为对照 ,研究了不同光质对黑曲霉产糖化酶及生长发育的影响。持续蓝光作用下 ,孢子萌发后菌丝较粗 ,菌丝细胞顶端膨大显著 ,菌丝细胞膜的通透性增加 ,残糖消耗快 ,孢子和孢子穗增大。在 3(4d时 ,蓝光下菌丝产糖化酶活力最高达 6 6 0 (30U mL ,比黑暗高出了 15. 4 % ,生物量增加了 4 9. 4 8% ,菌丝细胞可溶性蛋白含量提高了10 0. 5 6 % ,尤其是在开始产孢子的阶段 ,蓝光下黑曲霉产糖化酶活力、生物量有很大提高。研究表明 ,蓝光明显促进黑曲霉分生孢子发育和产孢阶段包括糖化酶在内的多种淀粉酶活力的迅速增加。

Pure blue(BL)or red light(RL) were obtained by filtering blue or red fluorescent lamp light through plastic filters. With the dark condition as control, the effect of BL and RL on the Aspergillus niger were studied including soluble protein content of mycelium, glucoamylase activity, the biomass as well as sporangiophore photomorphogenesis and vegetative spore development. Irradiation with BL and RL wholly promoted glucoamylase activity together with the rise of the soluble protein content and the mycelium growth. The glucoamylase activity of mycelia culture under blue light was enhanced 2.54 times than that of control. In the phases of developmental growth (young mycelia and sporangiophores), glucoamylase activity and relative conductivity ratio of mycelium were higher under blue light than under dark, the enhanced glucoamylase activity likely stemmed from development of macrosporangiophores of Aspergillus niger promoted by blue light. In addition, the increase of glucoamylase activity under blue light was highest at the stage of macrosporangiophores forming and conidiation, then declined owing to spores aging. As to young microsporangiophores under darkness the glucoamylase activity just relatively increased and declined rapidly with age. Different from control, irradiation with blue light at the time of 72h after inoculation was able to stimulate development of macrosporangiophore and conidium along with the rise of glucoamylase activity of Aspergillus niger.