中老年人骨髓间质干细胞的生物学特性研究

Biological Characteristics of Bone Marrow Mesenchymal Stem Cells in the Middle-aged and the Old People

目的:研究中老年人骨髓间质干细胞(MSCs)的生物学特性。方法:用Ficoll-Paque分离不 同年龄段供者MSCs体外培养,进行诱导分化,用流式细胞术检测表面标志,核型分析观察细胞染 色体的稳定性,裸鼠实验、软琼脂实验观察致瘤性。结果:中老年人每毫升骨髓分离的单个核细 胞量和MSCs增殖速度与青年人无显著差异;中老年人不同代次的MSCs诱导后具有向成骨细胞 和成脂细胞分化的能力;分离培养的MSCs表达CD44,不表达CD34;传至第10代核型未见异常, 未见致瘤性。结论:中老年人MSCs有较强的体外增殖能力和多向分化潜能,遗传背景稳定。

Objective: To investigate the biological characteristics of bone marrow mesenchymal stem cells(MSCs) derived from the middle-aged and the old people. Methods: MSCs of donors at different ages were harvested with Ficoll-Paque and cultured in vitro. MSCs were induced in vitro and assessed expression of cell surface antigen by FACS. The genetic stability was verified by karyotype analysis, and carcinogenic ability of the MSCs was also tested. Results: The number of mononuclear cells per mililter bone marrow and the growth rate had no distinction among donors at different ages. MSCs of different passages from the middle-aged and the old people could be differentiated into osteocytes and adipocytes after inducement. MSCs were positive for CD44 and negative for CD34. The karyotype was stable and carcinogenicity was not found in the tenth-passaged MSCs. Conclusion: MSCs derived from the middle-aged and the old people had no difference in proliferation and differentiation from the young, and the genetic background of MSCs was stable.