无透明带核移植技术

Nuclear Transfer Using Zona-free Oocytes

近几年,虽然体细胞核移植研究取得了巨大的成就,但是基本上沿用Willadsen最初的方法——去核、注核、融合和激活。而且所获得的克隆动物往往会出现很多问题如器官发育异常等,其中的原因不清。因此,人们试图革新原有的核移植方法,并建立了新的核移植方法——无透明带核移植法,以期提高核移植的效率及解决核移植中存在的问题。现对无透明带核移植技术的两种方法——反向核移植法和手工核移植法以及单个胚胎培养系统进行介绍。

Although somatic nuclear transfer (NT) achieved a lot of progress in recent years, the ways of NT was still the same to the Willadsen’s method: enucleation, injection, fusion and activation. Cloning animals showed many problems such as abnormally developing organs, but the reason was unclear. Therefore, it is neces- sary to innovate the ways of NT. A novel NT method that employing zona-free oocyte has been developed in order to improve the efficiency and solve exited problems of NT. This paper introduces two methods of zona-free NT: the reverse cloning and handmade cloning as well as the single embryo culture system.