摘要
目的探讨辣椒素毁损感觉神经生长的情况下,对电针调控胃运动的影响作用及意义。方法电针刺激经辣椒素干预大鼠足三里穴,采用免疫组织化学方法及电生理的方法,检测原癌基因c fos在中枢延髓的孤束核(NTS)及迷走神经背运动核(DMV)中的表达以及中枢延髓的孤束核及迷走神经背运动核中神经元细胞放电频率的变化情况,同时采用浆膜法观察胃电的变化情况。结果辣椒素针刺组胃电的波幅和频率与生理盐水针刺组存在显著性差异(P <0 .0 1) ,生理盐水针刺组胃电的波幅和频率与单纯生理盐水组胃电的波幅和频率存在显著性差异(P <0 .0 1)。原癌基因c fos在孤束核及迷走神经背运动核中的表达,辣椒素针刺组阳性率与生理盐水针刺组存在显著性差异(P <0 .0 1) ,生理盐水针刺组阳性率与单纯生理盐水组存在显著性差异(P <0 .0 1)。辣椒素针刺组NTS与DMV内神经元细胞放电频率的变化与生理盐水针刺组存在显著性差异(P <0 .0 1)。而与辣椒素非针刺组及单纯生理盐水组未见有明显差异(P >0 .0 5 ) ;同时,生理盐水针刺组NTS与DMV内神经元细胞放电频率与单纯生理盐水组存在显著性差异(P <0 .0 1)。结论电针对胃运动具有调控作用,且电针对穴位的作用性质可能是一种伤害性刺激。
Objective To detect the effect and significance of capsaicin on gastric motor in electroacupuncture rat. Methods By using the radioactive immunoassay and electrophysiological methods, we observed the c-fos gene expression in the dorsal motor nucleus of the vagus nerve (DMV) and nucleus tractus solitary(NTS), and the change of electro-gastric graph(EGG) under acupoint Zusanli with electroacupuncture under the capsaicin injection in rats. Results The change of electrogastric graph of amplitude and frequency was significantly different in capsaisin group from control group when acupoint Zusanii was electroacupunctured.We observed the c-fos gene expression in DMV and NTS of without capsaicin group injection, was significantly different from capsaicin injection group.The electric activity of nurver cell in DMV and NTS was obviously different in capsaicin injection group from control group. Conclusion Electroacupuncture can regulate gastricmotility.The character of elctroacupuncture on acpoint is a kind of stimulation of damage.
出处
《胃肠病学和肝病学杂志》
CAS
2005年第2期129-133,共5页
Chinese Journal of Gastroenterology and Hepatology
基金
国家自然科学基金资助项目(No.39970888)
