HBV基因型和HBV核心启动子双点突变与肝损伤的关系探讨

The relationships between HBV genotypes and the double mutation in the basic core promoter (BCP) and liver damage

目的了解HBV基因型在荆州地区的流行病学状况;探讨HBV基因型和HBV核心启动子双点突变与肝损伤的相关性。方法采用PCR微板核酸杂交 ELISA技术,对临床诊断为不同程度的乙型肝炎患者血清中的HBVDNA进行基因分型和核心启动子双点突变检测。结果本地区基因型以B型和D型为主,分别为3 1 4%和2 4 4%。其它为A型3 5 % ,C型18 6% ,B、C混合型11 6% ,B、D混合型10 5 %。结论HBV基因型在本地区分布有其独特之处,HBV核心启动子双点突变在C型发生频率高于B型,有显著性差异,但与D型比较无显著性差异。HBV基因型及HBV核心启动子双点突变与肝损伤彼此间无明显相关性。肝损伤可能主要与机体免疫等相关,HBV变异仅起次要作用。

Objective To explore the epidemiological characteristics of HBV genotypes in Jingzhou city and the relationships between HBV genotypes and the BCP double mutation of HBV and liver damage.Methods HBV-DNA genotypes and the BCP double mutation were determined in sera from patients with different liver damage degrees by the techniques of polymerase chain reaction microplate nucleotide hybridization-enzyme linked immunosorbent assay (PCRmmh-ELISA).Results The genotypes in the area were predominant in type B and D, 31.4% and 24.4%, respectively. The others were A 3.5%,C 18.6%,the mixture of B and C 11.6% ,and the mixture B and D 10.5%.Conclusion Distribution of HBV genotypes is characteristic in our area.HBV genotype C is frequently higher than B in the BCP double mutation of HBV. There are significant differences.HBV genotypes and the BCP double mutation and liver damage are not significantly correlated with each other.Liver damage is probably associated with body immunity.HBV mutation maybe play a secondary role in Liver damage.