牙周治疗对血清IFN-γ及IL-4水平的影响

Changes of circulating IFN -γ, IL-4 in patients with chronic periodontitis before and after periodontal initial therapy

目的:探讨牙周基础治疗对牙周炎患者血清干扰素-γ(IFN-γ)及白细胞介素-4(IL-4)水平的影响。方法:检测慢性牙周炎患者牙周基础治疗前和治疗后4～6周内,牙龈探诊深度、出血指数;采用双抗体夹心ELISA法,通过绘制标准曲线求出标本中IFN-γ和IL-4的浓度,对检测结果进行t检验。结果:慢性牙周炎患者血清IFN-γ、IL-4显著高于对照组(P<0.01);慢性牙周炎患者经牙周基础治疗后,其探诊深度、出血指数、血清IFN-γ浓度均显著降低(P<0.01),血清IL-4浓度无显著变化(P>0.05)。结论:IFN-γ、IL-4与牙周组织炎症的发病有关,控制牙龈炎症可降低血清IFN-γ水平,但IL-4浓度不因牙龈炎症的控制而下降。

PURPOSE: To investigate the effect of periodontal initial therapy on circulating blood serum interferon-gamma (IFN-γ)and interleukin-4(IL-4) in patients with chronic periodontitis. METHODS: 15 patients with chronic periodontitis and 15 healthy people were selected. Circulating blood serum IFN-γ and IL-4 were measured using double antibody sandwich ELISA ; Student's t test was used for comparing sulcus bleeding index (SBI), probing depth(PD), and circulating IFN-γ, IL-4 in patients with chronic periodontitis that were assessed respectively before and 4 to 6 weeks after periodontal initial therapy. RESULTS: The level of blood serum IFN-γ, IL-4 in patients with chronic periodontitis was significantly higher than the control group (P <0.01); After periodontal initial therapy, SBI, PD, and circulating IFN-γ concentration were reduced significantly(P<0.01); IL-4 concentration had no significant difference (P>0.05). CONCLUSIONS: Blood serum IFN-γ, IL-4 were associated with chronic periodontitis; The control of gingival infection may reduce blood serum IFN-γconcentration; IL-4 concentration was not reduced with the control of gingival infection.Periodontal initial therapy