微波萃取、高效液相色谱法测定番石榴叶中的齐墩果酸和熊果酸

HPLC Determination of Oleanolic Acid and Ursolic Acid in Psidium Guava Leaves Using Microwave-assisted Extraction for Sample Pretreatment

建立了微波萃取、高效液相色谱测定番石榴叶中齐墩果酸和熊果酸的方法.采用分式析因设计实验考察了微波萃取参数的影响,番石榴叶粒度和萃取时间是影响齐墩果酸和熊果酸的微波萃取产率的最重要因素.优化的微波萃取条件如下:60～80 mesh番石榴叶、10 mL乙醇、80℃和10 min.微波萃取与索氏萃取得到的齐墩果酸和熊果酸产率一致,然而,前者仅需10 min,后者则需要4 h.

A MAE-HPLC method for the determination of oleanolic acid and ursolic acid in guava leaves was developed. A fractional factorial experimental design allowed for the determination of the effects of extraction variables. The most important variables were the sample particle size and the extraction time.Optimized MAE conditions included 60~80 mesh of guava leaves, 10 mL of ethanol, 80℃ and 10 min. The method was accurate and superior to the Soxhlet method in saving much extraction time.