抗人精浆蛋白McAb的制备及其特性研究

PREPARATION AND CHARACTERISTICS OF SPERAMIMOBILIZING MONOCLONAL ANTIBODY TOHUMAN SEMINAL PLASMA PROTEIN

本文报道用杂交瘤技术建立了２株分泌抗人精浆蛋白单克隆抗体的细胞株Ｄ１和Ｃ１２。Ｄ１株分泌的抗体为ＩｇＭ，Ｃ１２株为ＩｇＧ３。这两株均能与正常人精浆和无精子症患者的精浆产生特异性免疫反应。Ｄ１株单抗具有补体依赖性制动人精子的作用，间接免疫荧光试验表明，它能结合到人精子颈部和顶体后区。这充分说明２株单抗是特异性抗人精浆的，Ｄ１是精子制动抗体，其相应的精子抗原属于精子外套抗原成分之一。

Two strains of monoclonal antibodies(D1 and C12)to human seminal plasma were produced by the use of the hybridoma techniquOne was IgG3(D1)and the other IgM(C12)subtype.These McAbs showed both positive reaction to the seminal plasma in normal subjects as well as in patients with azoospermia and negative reaction to the serum of normal human in ELISA;Only McAb D1 had a positive sperm-immobilization effect.With indirect immunofluorescent antibody technique it was shown that D1 McAb was bound to the neck and postacrosome of the sperm The results indicated that McAb D1 was a human immobilizing monoclonal antibody to human sperm and the D1 antigen was SCA.