RCS大鼠病变发育过程中视网膜神经节细胞形态学变化的研究

Study on morphological characteristics in the development of the retinal ganglion cells of RCS rats

目的 研究皇家外科学院大鼠(royalcollegeofsurgeonrat,RCS)病变发育过程中视网膜神经节细胞(retinalganglioncells ,RGCs)形态学变化。方法 取RCS大鼠、RCS rdy+ 大鼠3、5、7、9、11周龄视网膜,固定后行视网膜平铺片,甲酚紫染色,使用LEICADMTRET显微镜及自带的LEICAQWIN系统软件,观察视网膜神经节细胞层细胞数量总数及RGCs亚群数量上的变化。结果 RCS大鼠病变发育过程中,其节细胞层中细胞横径≥12 μm的细胞在数量上,7、9、11周龄段相较与3周龄段出现显著减少(P <0 0 1)。相同周龄段上的视网膜节细胞层中,RCS大鼠与RCS rdy+ 大鼠在横径≥12 μm的细胞数量上相互比较,可见RCS大鼠从5周龄段开始相较于RCS rdy+ 大鼠出现显著减少(P <0 0 5 ) ,7、9、11周龄段相较与RCS rdy+ 大鼠减少更为显著(P <0 0 1)。但是节细胞层细胞总数及横径≥9μm的细胞数量上,RCS大鼠自身发育前后对照以及与RCS rdy+ 大鼠相比较没有显著差异。结论 RCS大鼠视网膜色素变性过程中,随着光感受器细胞的变性和缺失,虽然视网膜节细胞层细胞在整体数量上没有明显变化,但是RCS大鼠视网膜病变发育早期,其节细胞层中胞体横径≥12 μm的RGCs在数量上开始出现明显缺失。

Objective To study the morphological diversity of retinal ganglion cells in the pathological development of retinitis pigmentosa in royal college of surgeon (RCS) rats. Methods The retinal whole mount preparations of RCS and RCS-rdy + rats of 3, 5, 7, 9, 11 weeks were stained by cresyl violet to count the number of the different subpopulation of the RGCs and the number of all the cell of the entoretina. Results In the development of the retina of the RCS rats, there was significant difference of the amount of the cell diameter more than 12 μm in the ganglion cell layer between the postnatal 3 weeks and the postnatal 7, 9, 11 weeks. There was also significant difference of the amount of the cell diameter more than 12 μm in the ganglion cell layer between RCS and the RCS-rdy + rats of different ages. But there was no significant difference between all the cell of the entoretina and the cell diameter more than 9 μm. Conclusion In the pathological development of retinitis pigmentosa, accompanied with the loss of the sensory retinal neuron, some kind of the subpopulation of the retinal ganglion cells have lost, although the amount of all the cell of entoretina was stable.