硫普罗宁对梗阻性黄疸大鼠肾功能保护作用的研究

目的探讨硫普罗宁对实验性梗阻性黄疸大鼠肾功能的保护作用及作用机理。方法SD大鼠胆总管结扎后分2组,每组20只,术后分别用1ml硫普罗宁或生理盐水腹腔注射。另设假手术组20只:分别于术后10d、20d(每组10只)心脏取血测定血清超氧化物歧化酶(SOD)、丙二醛(MDA)含量,同时测定血清肌酐(Cr)、尿素氮(BUN)和直接胆红素(DB)浓度,并对肾脏行光镜下病理形态学观察。结果血清MDA浓度在梗阻10d即升高,且随胆道梗阻时间延长进一步升高,SOD在梗阻10d即下降,同时伴有血清Cr、BUN、DB的升高和肾脏病理形态学的进行性改变。硫普罗宁治疗组MDA显著低于对照组,SOD显著高于对照组,并能改善肾组织病理形态。结论大鼠梗阻性黄疸氧自由基损害是肾损伤的原因,硫普罗宁通过降低血清MDA水平并提高SOD活性,对梗阻性黄疸大鼠的肾脏起保护作用。

Objective To evaluate the protective effects and mechanism of Tiopronin for renal function of rats with obstructive jaundice(OJ). Methods 40 rats with bile ducts ligated were divived into NS group(NS)and Tiopronin group at random. The rats were administered 1ml Saline and 1ml Tiopronin since the second day after bile duct ligation.20 rats were show-operation. The levels of superoxide dismutase(SOD) and malondialdehyde(MDA) were measured in rats(n=10) for 10 and 20 days postoperatively. Meanwhile, the levels of serum bilirubin(DB) and BUN and Cr were also determined in all rats. Renal histological changes were observed with optical microscopy. Results The level of MDA as well as those of serum DB, BUN and Cr in all OJ subgroups were continually elevated. The level of SOD in OJ rats was lower than that in sham-operation group. The level of MDA in Tiopronin group was lower than that in NS group, and the SOD content was higher than that. In NS group ameliorated the pathological changes of the renal tissue. Conclusion In rats with obstructive jaundice, Tiopronin may ameliorate renal function by antioxidation.