115例汉族骨髓供者HLA-Cw位点基因多态性的研究

Study of HLA-Cw Polymorphism in the 115 Han Bone Marrow Registry Donors

目的 调查汉族骨髓供者中常见的HLA Cw等位基因。方法 对115例骨髓捐献者采用PCR SSP方法进行HLA Cw低分辨和高分辨分型。结果 115例汉族骨髓捐献者中,HLA Cw位点基因频率分布由高至低依次为Cw 0 3(0 .2 5 98)、Cw 0 1(0 .2 14 2 )、Cw 0 7(0 .1978)、Cw 0 8(0 .115 4 )、Cw 12 (0 .0 5 82 )、Cw 14 (0 .0 399)、Cw 15 (0 .0 399)、Cw 0 6(0 .0 399)、Cw 0 4 (0 .0 30 9) ;进一步检测到16种等位基因,其中Cw 0 10 1、Cw 0 30 4、Cw 0 30 2、Cw 0 4 0 1、Cw 0 6 0 2、Cw 0 702、Cw 0 80 1、Cw 12 0 2、Cw 14 0 2、Cw 15 0 5、Cw 15 0 2为对应抗原最为常见的等位基因。结论 本研究在DNA水平上,提供了中国汉族人群中HLA Cw位点基因分布频率及常见等位基因资料。

Objective To analyse the allele frequencies of HLA-Cw locus in 115 Han bone marrow registry donors.Methods HLA-Cw locus were genotyped for 115 Han bone marrow registry donors by low-resolution and high-resolution PCR-SSP methods. Results The gene frequencies of HLA-Cw *03, *01, *07, *08, *12 , *14 , *15, *06, *04 were 0.259 8,0.214 2,0.197 8,0.115 4,0.058 2,0.039 9,0.039 9,0.039 9,0.030 9 respectively.Sixteen HLA-Cw alleles were detected in115Hanbonemarrowregistrydonors.HLA-Cw *0101,Cw *0304,Cw *0302,Cw *0401,Cw *0602,Cw *0801,Cw *120 2,Cw *140 2,Cw *150 5,Cw *150 2 were the most common alleles in relative antigens.Conclusion On DNA level,this study provide the data of HLA-Cw allele frequencies in Chinese Han population.