兽药单克隆抗体研究进展

Progress on Monoclonal Antibodies of Veterinary Drug

兽药大多属于小分子物质(半抗原),没有免疫原性,必须与载体交联成人工合成抗原(完全抗原)才能获得免疫原性,用人工合成的抗原来免疫动物制备兽药单克隆抗体。对兽药单克隆抗体的研究是随着兽药残留检测技术发展而开始的,人们已经在制备兽药单克隆抗体所需技术如兽药人工抗原合成的方法、选择合成兽药人工抗原的所需载体、对兽药结构的改造、免疫动物的注射方式、免疫程序、杂交瘤细胞的筛选及培养等方面进行一些研究,并成功地制备出十几种兽药单克隆抗体,且大多已应用于兽药免疫测定法(IAs)。目前仅有一种兽药单克隆抗体已应用于兽药免疫亲和色谱(IAC)。

The most veterinary drugs are small molecule’s substances.They don’t have the immunogenicity and cann’t be used as antigens to make monoclonal antibodies.If they aren’t connected with carriers.In order to analyse residues of veterinary drugs, the techniques of preparation of monoclonal antibodies against veterinary drugs were conducted,such as methods to prepare artificial antigens,how to choose proteins as carriers and change the strutures of veterinary drugs, injection means and how to choose or culture hybridoma cells.More than ten types of monoclonal antibodies against veterinary drug had been made. Most of them had been used in immunoassays,but only one of them had been used in immunoaffinity chromatography.