糙皮侧耳染色体计数和担孢子形成过程中细胞核行为的研究

CHROMOSOME NUMERATION AND BEHAVIOR OFNUCLEI DURING BASIDIOSPORE FORMATIONIN PLEUROTUS OSTREATUS

糙皮侧耳(Pleurotus estreatus)菌褶经有丝分裂阻断剂预处理、原生质体铺片和Giemsa染色并结合苏木精染色后,经过多次反复实验观察,证明糙皮侧耳的染色体条数为9(n=9);糙皮侧耳从菌褶分化完成到子实体完全成熟的过程中,不断有少量新的双核担子产生,发生核配直到释放担孢子。其减数分裂同步性不高。减数分裂后,4个子核分别进入4个担孢子中,留下中空的担子。

Cytological studies of chromosome numbers and basidiospore formation in Pleurotus ostreatus with light microscope were made at every three hour intervals from the completion of lamella differentiation to mature stage of fruiting body, by using propionic iron haematoxylin and Giemsa staining methods. It was found that some young binucleate basidia were produced during the course of fruiting body development. It took about 18 hours for basidia to develop from karyogamy to completion of meiosis. We found that chromosome number of P. ostreatus is 9 (n = 9) after careful observation at various stages of meiosis, especially at diakinesis. Four nuclei immigrated into four basidiospores respectively, leaving the basidia empty. Most of the basidia at tetrad stage were tetranucieate and a few were quinquenucleate.