实验性抑郁症大鼠缰核和海马BDNF基因表达

Changes of BDNF level expression in Hippocampal and Habenular nucles in experimental depression rats

目的:研究抑郁症大鼠缰核和海马BDNF基因的表达情况。方法:采用长期未预知应激刺激致大鼠抑郁症模型,运用open field和forcedswimmingtest法观察大鼠行为学变化。运用免疫组化法检测缰核和海马BDNF基因的表达。结果:与正常对照组相比,抑郁症模型组大鼠海马和缰核均表现为BDNF阳性细胞数量明显减少。结论:实验性抑郁症大鼠缰核、海马BDNF基因表达水平降低。

Objective:To observe the change of BDNF level expression in experimental in hippocampal and habenular nucles in experimental depression rats.Methods:The depression model of rats was established by chronic unpredictable mild stress. Openfield test was performed to detect the behavior of rats. Immunohistochemistry was used to observe the changes of BDNF level.Results:Compared to the control group,the number of depressed animals was much less than that of normal animals.Conclusion:The level of BDNF decrease significantly in experimental depressive model of rats.