儿童再生障碍性贫血干细胞因子及受体的表达

Expression of SCF in Serum and c-kit Receptor on Bone Marrow Mononuclear Cells of Children with Aplastic Anemia

目的探讨造血干细胞因子及其受体表达在儿童再障发病机制中的作用。方法采用Elisa法测定血浆中干细胞因子(SCF)水平;免疫细胞化学方法检测骨髓单个核细胞(BMMNC)中干细胞因子受体c-kit蛋白(CD117)的表达。结果SCF在慢性再障组(CAA)、严重再障组(SAA)、正常儿童组分别为363.55±134.31pg/ml、326.16±108.29pg/ml、468.24±147.21pg/ml,CAA、SAA组与正常对照组比较差异有显著性(P均<0.01);c-kit阳性细胞表达值(A)在慢性再障(CAA)、严重再障(SAA)和正常对照组分别为0.52±8.81、0.46±2.18、0.58±7.94,CAA、SAA组与正常对照组比较差异无显著性(P均>0.05)。结论再障患儿血浆中SCF水平低于正常对照组;再障患儿BMMNC中c-kit受体表达与正常对照组之间差异无显著性,提示再障骨髓造血衰竭并非c-kit蛋白低表达所致,可能与SCF浓度降低有关。

Objective To explore the relationship between the expression of stem cell factor(SCF) and its receptor (c-kit) and the pathogenesis of pediatric aplastic anemia. Methods SCF was detected by Elisa assay; c-kit protein(CD117) in BMMNC by immunocytochemistry. Results The levels of SCF in serums of children with CAA,SAA,normal controls were (363.55±134.31)pg/ml、((326.16)±108.29) pg/ml、(468.24±147.21) pg/ml, there was especially significant difference between children with CAA,SAA,and normal controls (P<0.01); the values (A) of c-kit protein expression in CAA,SAA,normal controls were 0.52±8.81,0.46±2.18,0.58±7.94, respectively, there were no significant difference among CAA,SAA and control group (P>0.05). Conclusion The levels of SCF was significantly lower in children with CAA,SAA than that in normal controls; there was no significant difference between the c-kit expression of BMMNC in children with CAA,SAA and in normal controls,which suggested that the hematopoiesis failure of pediatric aplastic anemia is not attributed to low expression of c-kit protein and is probably related to low level of SCF in serum.