松材线虫和拟松材线虫的PCR快速检测

A rapid method to detect Bursaphelenchus xylophilus and B.mucronatus by PCR

利用PCR技术对松材线虫Bursaphelenchusxylophilus与拟松材线虫B .mucronatus的rDNA的ITS1区和5 8S区核甘酸序列扩增.根据松材线虫与拟松材线虫的ITS1序列区别,分别设计出松材线虫和拟松材线虫的特异性引物,实现了单条活的或FG(φ为4 %的甲醛)固定的松材线虫和拟松材线虫的快速检测.

A polymerase chain reaction(PCR) was used to amplify ribosomal DNA containing 5.8 S gene and internal transcribed spacer region 1(ITS1). According to the difference between the sequences of internal transcribed spacer region 1(ITS1) of Bursaphelenchus xylophilus and B.mucronatus, the species of specific primers were designed respectively for the two species nematodes. A single nematode, living or preserved in formalin, could be detected rapidly, and B.xylophilus and B.mucronatus were distinguished as well. This method would be useful for rapid detection of nematodes.