摘要
采用蛋白质连接技术合成玉米赤霉烯酮抗原，免疫Ｂａｌｂ／ｃ鼠，通过淋巴细胞杂交瘤技术建立六株分泌抗玉米赤霉烯酮的单克隆抗体杂交瘤细胞株。间接酶联免疫吸附试验测定细胞上清抗体效价为１：２０８４（４Ｈ８）、１：２５６（６Ｈ９、４Ｈ３、２Ｈ５、２Ｃ８）１：１６（３Ｆ１０）；腹水抗体效价为１０＾９（４Ｈ３、４Ｈ８）、１０＾８（２Ｈ５）、１０＾７（６Ｈ９）、１０＾５（３Ｈ１０）。
Six stabilized hybridoma cell lines secreting anti-zearalenone (ZEN) monoclonal antibodies (IgG) were produced by fusing the mouse cells (SP2/0) with splenocytes isolated from Balb/c mice. The Balb/c mice were previously immunized with ZEN-bovine serum albumin (BSA) conjugation which was prepared by ZEN-oxime with BSA. An indirect competitive ELISA was used to detect ZEN by anti-ZEN McAb. Cross reactivity of these antibodies with zearalenol was 1.3-9.0%. The sensitivity of CI-ELISA for ZEN was 0.3-0.8ng/ml. Average recovery rates of ZEN spiked (25-500ng/g) corn, feed and wheat were 105%, 103% and 90%, respectively. Mean coefficients of variation were 3.8%, 15.2% and 12.7% for interwell and 5.8%, 6.8% and 2.8% for interassay, respectively.
出处
《真菌学报》
CSCD
北大核心
1994年第4期303-309,共7页
基金
国家自然科学基金
