甘氨酰谷氨酰胺对猪离体骨骼肌卫星细胞增殖和分泌IGF-1的影响

The effects of glycyl-glutamine on the proliferation of in vitro cultivated procine skeletal muscle satellite cells and medium IGF-1 level

为探讨甘氨酰谷氨酰胺(Gly Gln)对猪离体骨骼肌卫星细胞增殖和分泌IGF 1的作用,取10日龄仔猪的半腱肌和背最长肌,将分离出的半腱肌卫星细胞和背最长肌卫星细胞分别按2×10 5mL-1密度接种于培养板上,采用浓度为0 6、1 2和2 4mmol/L的Gly Gln处理离体培养的猪半腱肌和背最长肌卫星细胞,观察Gly Gln对离体骨骼肌卫星细胞增殖和分泌IGF 1的影响.结果表明:用上述浓度的Gly Gln处理细胞,对离体培养的猪半腱肌卫星细胞的增殖有显著促进作用(P <0 0 5 ) ,而对离体培养的猪背最长肌卫星细胞的增殖有极显著的促进作用(P <0 0 1) ;同时发现:添加0 6mmol/LGly Gln对猪背最长肌卫星细胞分泌IGF 1有显著的促进作用,提示:不同浓度的Gly Gln对猪离体骨骼肌卫星细胞的增殖均有促进作用,但不同部位的骨骼肌卫星细胞对Gly

The purpose of this experiment was to explore the effects of glycyl-glutamine on the in vitro cultivated procine skeletal muscle satellite cells. The satellite cells were separated from semitendinosus muscle and longissimus dorsi of 10-day-old male healthy piglet and inoculated on cell-culture plate with a concentration of 2×10~5 mL^(-1). The effects of glycyl-glutamine on proliferation and medium IGF-1 level of in vitro cultivated porcine skeletal muscle satellite cells were observed. The results showed that the addition of glycyl-glutamine at 0.6, 1.2 and 2.4 mmol/L had distinct stimulating effect on the proliferation of satellite cells, and the addition of glycyl-glutamine at 0.6 mmol/L could significantly improve the secretion IGF-1 from the longissimus dorsi satellite cells.