摘要
利用改良的CATB方法提取大豆(Glycinemax)种子基因组DNA。以大豆内源ScII基因做对照,采用MPCR(MultiplexPolymeraseChainReaction, MPCR)技术从进口大豆中检测出35S启动子、NOS终止子和EPSPS耐除草剂基因3种转基因成分。
The DNA was successfully extracted from soybean(Glycine max) seeds by a modified CTAB method. Three transgenic elements, 35S promoter, NOS terminator and EPSPS (5-enolpyruvylshikimate-3- phosp-hate synthase), were detected in the imported soybean seeds by MPCR(Multiplex Polymerase Chain Reaction,MPCR) with the ScII as the control.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2005年第2期262-264,269,共4页
Acta Agriculturae Universitatis Jiangxiensis
基金
福建省重大国际合作项目(2001I002)
948项目"甘蔗良种和技术引进与推广"(2003-Q06)
863高新技术发展计划课题(2001AA241191)
关键词
MPCR
转基因
大豆
检测
MPCR
transgenic
soybean(Glycine max)
detection
