利用RAPD标记进行杉木无性系的识别

A Study on Identifying Clones of Cunninghamia lanceolata (Lamb) Hook by RAPD Molecular Markers

针对目前杉木无性系管理和利用中存在识别困难的问题,利用RAPD标记技术进行杉木无性系的识别研究,19个S系列随机引物对8个杉木无性系的扩增结果显示:共得到157条DNA扩增带,平均每个引物产生8.26条带,其中91条带表现为多态性,占总带数的57.96%,扩增片段长度为200～2500bp,8个杉木无性系间表现了较丰富的DNA多态性;利用5个引物的13个分子标记完全可以对8个不同杉木无性系进行识别,谱带清晰,因此利用RAPD技术进行杉木无性系识别是可行的.

Eight clones of Cunninghamia lanceolata (Lamb) Hook were identified and analyzed by RAPD molecular markers for their utilization and management. The PCR results of nineteen primers are as follows:one hundred and fifty-seven bands are obtained, ninety-one bands of them are polymorphic, the lengths of the segments are 200~2500bp and thirteen polymorphic markers (bands) under five primers can identify eight clones of C. lanceolata(Lamb)Hook completely. So RAPD molecular markers can be used to identify clones of C. lanceolata (Lamb) Hook.