bcl-2、bax、CD57在乳腺髓样癌中表达及其临床意义的研究

Studies of Clinical Significance of Expression of bcl-2、bax and CD57 in the Medullary Breast Carcinoma

目的:探讨细胞凋亡相关基因bcl- 2、bax蛋白及CD5 7在乳腺典型髓样癌、不典型髓样癌和硬癌中表达的临床意义。方法:通过TUNEL法检测三型乳腺癌中凋亡细胞数量及分布比例的差异,并采用S- P法检测三型乳腺癌组织中bcl- 2、bax蛋白及癌周浸润的淋巴细胞中CD5 7抗原表达阳性率的差异。结果:定量测定三型乳腺癌细胞凋亡均数、面密度均数、面数密度均数及实际面数密度均数4项指标,两型髓样癌显著高于硬癌(P<0 .0 5 ) ;检测三型乳腺癌bcl- 2、bax蛋白及癌周浸润的淋巴细胞CD5 7抗原阳性率,bcl- 2与CD5 7髓样癌显著高于硬癌(P<0 .0 5 ) ,bax在三型乳腺癌中差异无显著性(P>0 .0 5 )。结论:髓样癌尤其是典型髓样癌临床预后比硬癌好,与细胞凋亡水平高有关;凋亡相关基因bcl- 2 /bax的失衡及A-

Objective To explore clinical significance of expression of apoptosis correlationalgenes-bcl-2?bax protein and CD57 in breast typical medullar carcinoma,atypicalmedullar carcinoma and scirrhous carcinoma.Methods The specimens from 61 cases of breast cancer were detected by TUNEL and S-P immunohistochemical method.Results The mean of apoptosis cells?area-density?area-numerical-density and realarea-numerical-density of medullary carcinoma were significantly higher than that of scirrhous carcinoma(P<0.05).The expression of bcl-2 and CD57 protein in medullarycarcinoma were significantly higher than that of scirrhous carcinoma(P<0.05).The expression of bax protein shows no difference in three kinds of breast carcinoma(P>0.05).Conclusions Prognosis of medullary carcinoma especial typical medullary carcinoma is better than that of scirrhous cancinoma,which correlate with their higherapoptosis level;Apoptosis correlational gene-bcl-2/bax off balance and faction of NK-cellmay play important role in prognosis of medullary breast carcinoma.