亚胺培南-EDTA纸片增效法检测非发酵菌的金属β-内酰胺酶

Detection of metallo-β-lactamase produced from non-fermenting bacilli by using imipenem-EDTA disk method

目的为了解临床分离的非发酵菌(NFB)产金属β-内酰胺酶(MBL)阳性率。方法采用MH琼脂扩散法,以加与不加EDTA亚胺培南纸片的抑菌环直径差异确定MBL的产生。结果484株待测NFB菌株中,MBL阳性率分别是:嗜麦芽窄食单胞菌为100.0%、铜绿假单胞菌为49.0%、洋葱伯克霍尔德菌为63.9%、鲍曼不动杆菌为7.5%、脑膜败血黄杆菌为94.1%、产吲哚黄杆菌为80.9%、少动鞘胺醇单胞菌为13.3%。结论临床分离的NFB存在较高的MBL阳性率,应采取有效措施防止产MBL菌的进一步扩散。

Objective To study the prevalence of metallo-β-lactamase(MBL) produced from non-fermenting bacilli(NFB) separated from clinical specimens. Methods The MBL-producers were detected by comparing the inhibition zones using imipenem containing EDTA or not in the MH agar. Results Among 484 tested strains of NFB, the MBL-producing strains were 100.0% in S.maltophilia, and with 49.0% of P.aeruginosa, 63.9% of B.cepacia, 7.5% of A.bauamanii, 94.1% of F.meningosepticum, 80.9% of F.indologenes, 13.3% of S. paucimobilis, respectively. Conclusions The NFB isolated from clinical specimens present a higher MBL-producing prevalence, so an efficient measures should be taken to prevente further spread of MBL-producing NFB.