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VEGF-A及VEGF-C mRNA在缺氧缺血性脑损伤新生大鼠脑组织中的表达 被引量：3

The expression of VEGF-A and VEGF-C mRNA hi brain of neonate rats with hypoxic-ischemic brain damage
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摘要 目的探讨血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)A和CmRNA在正常生后7日龄Wist ar新生大鼠和缺氧缺血性脑损伤(hypoxicischemicbraindamage,HIBD)后新生大鼠脑组织中的表达。方法应用原位杂交技术,通过正常生后7日龄Wistar新生大鼠HIBD动物模型,检测VEGF A和VEGF CmRNA在正常生后7日龄Wistar新生大鼠和缺氧缺血(hypoxic ischemic,HI)后不同时间点新生大鼠脑组织中的表达。结果VEGF A和VEGF CmRNA在正常生后7日龄新生Wistar大鼠脑组织中即有表达,生后第8天时达高峰,然后逐渐下降并呈低水平表达,持续至生后第35天(HI后28d);HIBD后新生大鼠脑组织中的VEGF A和VEGF CmRNA于HI后2h左右开始表达增强,HI后12h左右达高峰,其中VEGF CmRNA持续至HI后72h,而VEGF AmRNA持续至HI后14d。结论VEGF A和VEGF CmRNA在正常生后7日龄Wistar新生大鼠和HIBD后新生大鼠的脑组织中均有表达,但都以VEGF AmRNA的表达为主。
作者 梁云梅 王华
机构地区 中国医科大学附属第二医院
出处 《小儿急救医学》 2005年第2期134-136,i001,共4页 Pediatric Emergency Medicine
基金 辽宁省博士启动基金资助项目(200110255) 国家自然科学基金资助项目(30270488)
关键词 VEGF MRNA 新生大鼠 缺氧缺血性脑损伤
分类号 R722.1 [医药卫生—儿科]
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参考文献10

  • 1Waltenberger J, Claesson Welsh L, Siegnahn A, et al. Different signal transduction properties of KDR and Flt-1, two receptor of vascular endothelial growth factor [J]. J Biol Chem, 1994, 269(43): 26988-26995.
  • 2Kroll J, Waltenberger J. The vascular endothelial growth factor receptor DKR activates multiple signal transduction pathways in porcine aortic endothelial cells [J] .J Biol Chem, 1997, 272(51):32521-32527.
  • 3张天飞,蒋登金,郭光金.血管内皮生长因子与血管生成的研究进展[J].局解手术学杂志,2003,12(4):296-298. 被引量：6
  • 4Alon T, Hemo I, Itin A, et al. Vascular endothelial growth factor acts as a survival factor for newly formed retinal vessels and has implications for retinopathy of prematrity [J]. Nat Med, 1995, 1(10): 1024-1028.
  • 5Bemjamin LE, Keshet E. Conditional switching of vascular endothelial growth factor (VEGF) expression in tumors:induction of endothelial cell shedding and regression of hemangioblastomalike vessels by VEGF withdrawal [J]. Proc Natl Acad Sci USA,1997, 94(16) :8761-8766.
  • 6Chen JL, Zhang ZG, Li Y, et al. Intravenous administration of human bone marrow stromal cells induces angiogenesis in the ischemic boundary zone after stroke in Rat [J]. Circulation Res,2003,4 (4): 692-699.
  • 7Mu D, Jiang X, Sheldon RA, et al. Regulation of hypoxia-inducible factor lalpha and induction of vascular endothelial growth factor in a rat neonatal stroke model [ J ] ,J Neurobiol Dis, 2003,14(3):524-534.
  • 8Bernaudin M, Tang Y, Reilly M, et al. Brain Genomic Response following Hypoxia and Re-oxygenation in the Neonatal Rat [J]. J Biol Chem, 2002, 277(42) :39728-39738.
  • 9Ognnshola OO, Stewart WB, Mihalcik V, et al. Neuronal VEGF expression correlates with angiogenesis in postnatal developing rat brain [J]. Brain Res Dev Brain Res, 2000, 119(1): 139-153.
  • 10Kumasaka T, Seyame K, Mitani K, et al. Lymphangiogenesis in lymphangioleiomyomatosis: It Implication in the progression of lymphangioleiomyomatosis [ J ]. Am J Surg Pathol, 2004, 28(28): 1007-1016.

二级参考文献16

  • 1Benjamin LE, Hemo I, Keshet E, et al. Plasticity window for blood vessel remodelling is defined by pericyte coverage of the preformed endothelial network and is regulated by PDGF-B and VEGF[J]. Development, 1998, 125(9): 1591-1598.
  • 2Pettersson A, Nagy JA, Brown LF, et al. Heterogeneity of the angiogenic response induced in different normal adult tissues by vascular permeabillity factor/vascular endothelial growth factor[J]. Lab Invest, 2000, 80(1): 99-115.
  • 3Holash J, Maisonpierre PC, Compton D, et al. Vessel cooption,regression, and growth in tumors mediated by angiopoietins and VEGF[J]. Science, 1999, 284(5422): 1994-1998.
  • 4Zagzag D, Hooper A, Friedlander DR. In Situ expression of angiopoietins in astrocytomas identifies angiopoietin-2 as an early marker of tumor angiogenesis[J]. Exp Neurol, 1999, 159(2):391 - 400.
  • 5Ferrara N, Alitalo K. Applications of angiogenic growth factors and their inhibitors[ J]. Nature Med, 1999,5(11) : 1359 - 1364.
  • 6Dale NW, Yancopoulos GD. Growth factors acting via endothelial cell - specific receptor tyrosine kinases: VEGFs, angiopoietins and ephrins in vascular development[ J ]. Genes Dev, 1999, 13 (9) :1055- 1066.
  • 7Risau W. Mechanisms of angiongenesis [ J ]. Nature, 1997, 386(6626) : 671 - 674.
  • 8Socker S,Takashima S,Miao HQ,et al. Neuropilin-1 is expressed byendothelial and turmr cells as an isform-specific receptor for vascular endothelial growth factor[J]. Cell, 1998, 92(6): 735-745.
  • 9Hiratsuka S, Minowa O,Kuno J,et al. Flt-1 lacking the tyrosine kinase domain is sufficient for normal development and angiogenesis in mice[J]. Proc Nail Acade Sci USA, 1998, 95( 16): 9349-9354.
  • 10Taipale J. Makinen T, Arighi E, et al. Vascular endothelial growth factor receptor-3[ J]. Curt Top Microbiol immunol, 1999,237(1):85-96.

共引文献5

同被引文献45

  • 1李海燕,张祥建,张丽英,胡书超,祝春华,刘春燕.实验性脑出血大鼠脑组织血管内皮生长因子的表达及意义[J].中国全科医学,2004,7(15):1046-1048. 被引量：16
  • 2沈盈,俞惠民.一氧化氮及诱导型一氧化氮合酶与早产儿脑室周围白质软化[J].国际儿科学杂志,2006,33(1):55-57. 被引量：2
  • 3Senger DR, Galli S J, Dvorak AM, et al. Tumor cell secrete a vascular permeability factor that promotes accumulation of ascitesfluid[J]. Sci- ence, 1983,219:983-985.
  • 4Ferrara N, Henzel WJ. Pituitaty follicular cells secrete a hepambinding growth factor specific for vascular endothelial cells[J]. Biochem Biophys Res Commun, 1989,161(2):851-858.
  • 5Tischer E, Mitchell R, Hartman T. The human gene for vascular en- dothelial growth factor. Multiple protein forms are encoded through alternative axon splicing[J]. J Biol chem,, 1991,266(18): 11947-54.
  • 6Jim KL, Mao XO, David A, et al. Vascular enfothelial growth factor: Sirect neuroprotective effect in vitro ischemia[J]. PANS, 2000,97(18): 10242-10247.
  • 7Sondell M, Lundborg G, Kanje M. Vascular enfothelial growth factor has neurothrophic activity and stimulates axonal growth,enhanceing cell survial and Schwann cell proliferation in the peripheral nervous system[J]. J Neurosci, 1999;19(14) 5731-5741.
  • 8Kunlin J, Yonghua Z, Yunjuan S, et al. Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo [J]. Proc Nayl Acad Sei USA, 2000,97:10242-10247.
  • 9Krum JM, Khaibullina A. Inhibition of endogenous VEGF impedes revascularization and astroglial proliferation: roles for VEGF in brain repair[J]. Exp Neurol, 2003,181 (2):241-258.
  • 10Hoehn BD, Harik SI, Hudetz AG. VEGF mRNA expressed in microvessels of neonatal and adult rat cerebral cortex [J]. Molecular Brain Research, 2002,101 ( 1-2): 103 - 108.

引证文献3

二级引证文献10

小儿急救医学
2005年 第2期

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