溃结康胶囊对TNBS诱导大鼠结肠炎治疗作用的研究

The Effects of Kuijiekang Capsule on Ulcerative Colitis Induced by TNBS in Rats

目的:探讨溃结康胶囊对TNBS诱导大鼠结肠炎的治疗作用。方法:制备TNBS结肠炎大鼠模型,实验设正常对照组、模型对照组、阳性药物对照组(柳氮磺吡啶, 0. 50g/kg)、溃结康给药组( 0. 64, 0 .32, 0 .16g/kg),采用灌胃方式给药,给药时间从制备模型6d后开始,连续12天。实验结束后观察大鼠结肠粘膜损伤指数(CMDI)、粪便隐血(OB)、髓过氧化物酶(MPO)活性和粘膜病理组织学情况,并检测结肠组织丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH- Px)含量;胸腺、脾脏称重。结果:溃结康可减轻TNBS结肠炎大鼠的CMDI和OB程度,降低MPO水平,可改善结肠粘膜病理损伤;并可明显对抗胸腺萎缩和脾脏肿大;同时溃结康可明显降低MDA含量,增加SOD和GSH- Px水平。结论:溃结康对TNBS诱导的大鼠结肠炎有良好的治疗作用,其机理可能与抗炎、免疫调节和抗氧化损伤有关。

Objective: To evaluate the effects of Kuijiekang Capsule(KJK) on rats colitis induced by TNBS. Methods: Rats with TNBS/ethanol-induced colitis were used and treated with KJK. The experimental animals were divided into 6 groups: control group, model group, SASP group(0.50 g/kg), KJK group(0.64,0.32,0.16 g/kg). The animals were administrated 0.5% carboxymethyl cellulose, SASP and KJK respectively (from the 6 th d after the establishment of ulcerative colitis model to the end of the experiment, 18d totally). At the end of the experiment, the colon mucosal damage index(CMDI), the activity of myelperoxidase(MPO) and the occult blood test(OB) in feces were observed, the mucosa pathohistology was measured and thymus and spleen of rats were weighed respectively. Meanwhile, the content of malondiadehyde(MDA), superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px) of colonic tissue were detected. Results: The remarkable effects of KJK at dosage of 0.64, 0.32 g/kg on TNBS-induced colitis were observed, the extent of CMDI and OB were decrased, MPO activeity of colonic tissue was reduced. The extent of atrophy of thymus gland and intumesce of spleen of rat were ameliorated. Meanwhile, the content of MDA was reduced, and the activities of SOD and GSH-Px were increased. Pathological histology results showed that KJK could alleviate the pathohistological lesion of the colon of rat. Conclusion: Treatment with KJK shows beneficial effects on the mucosal damage of rats colitis induced by TNBS/ethanol. The mechanism of the actions of KJK may relate to anti-inflammatory effect, immunoloregulation and anti-oxidation.