一株高产γ-亚麻酸丝状真菌菌株的发酵接种方式研究

Studies on Inoculation Types of γ-linolenic Acid Fermentation by a High-producing Filamentous Fungus Strain

从土壤中筛选到一株高产γ-亚麻酸(GLA)的丝状真菌,GLA占总脂肪酸的12.85%,菌丝含油量为19.70%。经鉴定为根霉属真菌。比较两种不同的接种方式发现,孢子接种发酵所得菌丝体干物质产量、GLA含量以及油脂含量分别比菌丝接种高25.98%、51.90%和43.87%。用菌丝制作液体种子二级发酵,其GLA含量可以达到孢子接种水平,但菌丝生物量仍然低于孢子接种组。

A filamentous fungi strain identified as Rhizopus sp., producing high γ-linolenic acid (GLA) was obtained. In its mycelium, GLA amounted to 12.85% of the fatty acid while oil amounted to 19.70% of the mycelia. Two inoculation types were compared. By the spore inoculation the biomass, GLA composition and oil content of the mycelia were respectively 4.17g/L, 18.41% and 28.34%. By using mycelia inoculation gots the results were 25.98%, 51.90%, 43.87% higher than those above respectively. Using the mycelium as the secondary inoculums the final GLA content could reach the same level as that of the spores'; but the biomass could not meet the level of the spore inoculation.