红车轴草异黄酮提取工艺的研究

Study on Extraction Technology of Trifolium pratense Isoflavones

通过单因素实验和正交实验对红车轴草异黄酮提取工艺中乙醇浓度、温度、料液比、提取时间、提取次数等影响异黄酮提取率的因素进行探讨,确定红车轴草异黄酮最佳提取工艺参数为:乙醇浓度80%,温度80℃,料液比1:15,提取3次,每次1h,异黄酮提取率可达91.90%。

Extraction technology of isoflavones in Trifolium pratense was studied by single factor and orthogonal experiment. Effects of ethanol-water solvent, extraction temperature, extraction time, extraction times and ratio of liquid to material on extraction rate of isoflavone were investigated. The optimum extraction conditions for Trifolium pratense isoflavones were established as follows: 80% ethanol (V/V) as solvent, ratio of solvent to material 15:1(V/W), reflux extraction at 80℃ for 3 times and 1h for each time. The extraction rate of isoflavones in Trifolium pratense was as high as 91.90%.