小麦抗白粉病基因Pm4三个STS标记的实用性分析

Practicability Analysis of Three STS Markers forthe Wheat Powdery Mildew Resistant Gene Pm4

根据已报道的序列,合成了3对STS引物4aF + 4aR、4a + 4b和4G + 4I ,对2 4个含已知抗白粉病基因的品种、3个含未知抗白粉病基因的品种和4个感病品种进行了分析。结果表明,4G + 4I和4aF + 4aR 2对引物,扩增产物稳定,均能够扩增出特异性较强、易于检测的目标片段STS4 70 和STS4 10 ,并且标记STS4 70 与Pm4a基因完全连锁。这2个STS标记联合使用,能够准确地检测Pm4基因,作为分子标记辅助选择手段,在小麦抗白粉病育种中具有重要的应用价值。

Based on the reported sequences, 3 pairs of STS primers 4aF+4aR,4a+4b and 4G+4I were synthesized and used to analyze 31 wheat lines including 24 known Pm-carrying lines, 3 unknown Pm-carrying and 4 susceptible ones. The result showed that the 2 pairs of primers 4G+4I and 4aF+4aR could amplify stably the expected fragments STS 470 and STS 410 with more specificity, which could be detected easily. And the marker STS 470 was completely linked to Pm4a gene. The combination of markers STS 470 and STS 410 will be able to detect accurately the Pm4 gene and play an important part in wheat breeding aimed to powdery mildew resistance.