N^6-环戊基腺苷治疗敌敌畏急性中毒的实验研究

Therapeutic efficacy of N^6-cyclopentyladenosime against acute dichlorvos poisoning

目的 研究腺苷受体激动剂N6 环戊基腺苷(CPA)对急性敌敌畏(DDV)中毒的疗效和作用机制。方法 小鼠和大鼠用DDV灌胃染毒后,经腹腔注射生理盐水、CPA、阿托品(At)、氯解磷定(PAM Cl) ,观察各组动物中毒表现和死亡结局,测定DDV染毒大鼠CPA治疗和未治疗组全血胆碱酯酶(ChE)活力及乙酰胆碱(ACh)含量。结果 (1)CPA等治疗组动物与未治疗组相比,中毒表现出现时间延迟、中毒程度减轻、存活率提高;(2 )CPA治疗组大鼠全血ChE活力[(0 .4 9±0 .0 5 )U/ml]和未治疗组大鼠[(0 .5 2±0 .0 4 )U/ml]均比对照组大鼠[(1.5 6±0 .15 )U/ml]明显降低,差异有统计学意义(P <0 .0 1) ,但治疗组与未治疗组的差异无统计学意义(P >0 .0 5 ) ;(3)CPA治疗组大鼠全血ACh含量[(2 0 4 .2 4±2 0 .4 8) μg/ml]比未治疗组大鼠[(2 30 .91±2 5 .6 1) μg/ml]明显降低,差异有统计学意义(P <0 .0 5 )。结论 CPA对急性DDV中毒有治疗作用。

Objective To study the therapeutic efficacy and mechanism of adenosine receptor agonist N 6-cyclopentyladenosine (CPA) against acute dichlorvos poisoning. Methods Soon after a certain doses of dichlorvos were given to mice and rats by gastrogavage,physiological saline,CPA,atropine or pralidoxime chloride were administered to different groups.Toxic signs and survival rate were recorded and cholinesterase(ChE) activities and acetylcholine(ACh)concentrations in whole blood were determined in treatment group with CPA and non-treatment group after dichlorvos was given to rats by gastrogavage. Results (1)The alleviated and delayed appearance of toxic signs as well as obvious prolongation of survival time was observed in CPA treatment group compared with non-treatment group.(2)ChE activities in both treatment group with CPA[(0.49±0.05)U/ml] and non-treatment group[(0.52±0.04)U/ml] were significantly lower than that[(1.56±0.15)U/ml] of the control group ( P < 0.01) ,but there was no significant difference between treatment group and non-treatment group ( P > 0.05) .(3)ACh concentration[(204.24±20.48)μg/ml] in whole blood of treatment group with CPA was significantly lower than that[(230.91±25.61)μg/ml] of non-treatment group( P <0.05). Conclusion CPA has therapeutic efficacy against acute dichlorvos poisoning,which is probably through the decrease in ACh concentration.